George Groves: Former WBA world super-middleweight champion retires
Groves, 30, lost his world title to fellow Briton Callum Smith in the World Boxing Super Series final in September.
It was his 32nd bout in an 11-year professional career and in announcing his retirement Groves said he "knew the desire to fight had left" him.
He said: "I don't want there to be a time where I'm 'too old' to box, or where injury retires me."
In a statement, Groves offered "a prayer" for Eduard Gutknecht, who suffered a brain aneurysm after their fight in 2016, leaving him unable to walk or talk.
"This fight brought home the realisation that boxing can have brutal consequences," Groves added. "After this, I truly felt like my fighting days were numbered. After winning the WBA world title I decided to only continue fighting while it felt necessary."